Alan Fast

Northwestern University, Bachelor of Sciences, 2010

Research Project
The focus of my research is engineering the common industrial anaerobe, Clostridium acetobutylicum, to fix CO2 using electrons from H2 or CO with the purpose of producing useful metabolites, such as butanol, butyrate, and acetate. To accomplish this, we are engineering the cells with an efficient, anaerobic carbon fixation pathway – the Wood-Ljungdahl pathway – that exists natively in organisms known as acetogens. This project involves the resolution of major fundamental and practical problems by using the modern molecular and synthetic biology tools at our disposal

Selected Publications
 Fast, A.G., Schmidt, E.D., Jones, S.W., Tracy, B.P. Acetogenic mixotrophy: novel options for yield improvement in biofuels and biochemicals production, Current Opinion in Biotechnology, 33:60-72 (2015)

Nicolaou, S.A., Fast, A.G., Nakamaru-Ogiso, E., Papoutsakis, E.T. Overexpression of fetA (ybbL) and fetB (ybbM), encoding an iron exporter, enhances resistance to oxidative stress in Escherichia coli. Applied and Environmental Microbiology, 79 (23): 7210-7219, (2013)

Fast, A.G. & Papoutsakis, E.T. Stoichiometric and energetic analyses of non-photosynthetic CO2-fixation pathways to support synthetic biology strategies for production of fuels and chemicals, Current Opinion in Chemical Engineering, 1:380-395 10.1016/j.coche.2012.07.005. (2012)
Al-Hinai, M.A., Fast, A.G., & Papoutsakis, E.T.  Novel System for Efficient Isolation of Clostridium Double-Crossover Allelic Exchange Mutants Enabling Markerless Chromosomal Gene Deletions and DNA Integration, Appl. Environ. Microbiol. 78:8112-8121, (2012)

Tracy B.P., Jones S.W., Fast A.G., Indurthi, D.C., & Papoutsakis, E. T. Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications, Current Opinion in Biotechnology23:364-381, ISSN 0958-1669, 10.1016/j.copbio.2011.10.008. (2012)
>Research Summary Keywords

  • Metabolic Engineering
  • Synthetic Biology
  • Biofuels
  • Carbon Fixation

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