{"id":149,"date":"2016-06-14T17:19:52","date_gmt":"2016-06-14T17:19:52","guid":{"rendered":"http:\/\/papoutsakis.org\/?page_id=149"},"modified":"2016-09-08T19:53:02","modified_gmt":"2016-09-08T19:53:02","slug":"synthetic-methylotrophy","status":"publish","type":"page","link":"http:\/\/papoutsakis.org\/?page_id=149","title":{"rendered":"Synthetic Methylotrophy"},"content":{"rendered":"<figure id=\"attachment_162\" aria-describedby=\"caption-attachment-162\" style=\"width: 300px\" class=\"wp-caption alignnone\"><a href=\"http:\/\/papoutsakis.org\/?attachment_id=162\" rel=\"attachment wp-att-162\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-162 size-medium\" src=\"http:\/\/papoutsakis.org\/wp-content\/uploads\/2016\/02\/Methylotrophy-300x125.jpg\" alt=\"Creating a synthetic methylotrophic E. coli\" width=\"300\" height=\"125\" srcset=\"http:\/\/papoutsakis.org\/wp-content\/uploads\/2016\/02\/Methylotrophy-300x125.jpg 300w, http:\/\/papoutsakis.org\/wp-content\/uploads\/2016\/02\/Methylotrophy.jpg 1080w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><figcaption id=\"caption-attachment-162\" class=\"wp-caption-text\">Creating a synthetic methylotrophic E. coli<\/figcaption><\/figure>\n<p>Methylotrophy describes the ability of microorganisms to utilize reduced one carbon\u00a0compounds, such as methane and methanol, as carbon and energy sources. The recent\u00a0discovery of large natural gas reserves has prompted considerable interest in utilizing\u00a0these compounds as substrates or co-substrates with sugars in industrial fermentation of\u00a0fuels and chemicals, as higher biomass and product yields are expected from these more\u00a0reduced substrates. Native methylotrophs represent poor industrial microorganisms since\u00a0many are strict aerobes, produce few metabolites and lack genetic engineering tools.\u00a0Therefore, the development of synthetic methylotrophy is of considerable interest.\u00a0Towards this end, our research is focused on engineering Escherichia coli as a platform\u00a0microorganism for methanol metabolism. Through metabolic and protein engineering\u00a0techniques, we aim to develop a methylotrophic E. coli strain capable of metabolizing\u00a0methanol into biomass and valuable chemical products.<\/p>\n<p><strong>Supported by the US DOE ARPA-E agency through contract no. DE-AR0000432.<\/strong><\/p>\n<p>Antoniewicz, M., Bennett, R. K., Falara, V., Fast, A., Gonzalez, J., Nicolaou, S., Papoutsakis, E. T.,<\/p>\n<p>Selected Publications:<\/p>\n<ul>\n<li>Sandoval, N. R., Whitaker, W. B. Synthetic methylotrophy to liquid fuels and chemicals. WO2015108777-A1.<\/li>\n<li>Whitaker WB, Sandoval NR, Bennett RK, Fast AG, Papoutsakis ET. 2015. Synthetic methylotrophy:\u00a0engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization.\u00a0Current Opinion in Biotechnology 33:165-75.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Methylotrophy describes the ability of microorganisms to utilize reduced one carbon\u00a0compounds, such as methane and methanol, as carbon and energy sources. The recent\u00a0discovery of large natural gas reserves has prompted considerable interest in utilizing\u00a0these compounds as substrates or co-substrates with sugars in industrial fermentation of\u00a0fuels and chemicals, as higher biomass and product yields are expected [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":69,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-149","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/papoutsakis.org\/index.php?rest_route=\/wp\/v2\/pages\/149","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/papoutsakis.org\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/papoutsakis.org\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/papoutsakis.org\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/papoutsakis.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=149"}],"version-history":[{"count":3,"href":"http:\/\/papoutsakis.org\/index.php?rest_route=\/wp\/v2\/pages\/149\/revisions"}],"predecessor-version":[{"id":208,"href":"http:\/\/papoutsakis.org\/index.php?rest_route=\/wp\/v2\/pages\/149\/revisions\/208"}],"up":[{"embeddable":true,"href":"http:\/\/papoutsakis.org\/index.php?rest_route=\/wp\/v2\/pages\/69"}],"wp:attachment":[{"href":"http:\/\/papoutsakis.org\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=149"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}