Systems and Synthetic Biological Engineering
Education
Research Focus
I work on implementing mathematical modeling to improve biomanufacturing of glycoproteins. Kinetic and stoichiometric models are used to study the effects of Critical Process Parameters (CPP) on Chinese Hamster Ovary cell metabolism. Models for site-specific N-linked glycosylation were built to study the effect of CPPs on site-specific N-linked glycosylation.
List Publication
Naik, H. M, Cai, X, Ladiwala, P, Reddy, JV, Betenbaugh, M. J, & Antoniewicz, M. R (2024). Elucidating uptake and metabolic fate of dipeptides in CHO cell cultures using 13C labeling experiments and kinetic modeling. Metabolic Engineering. doi:https://doi.org/10.1016/j.ymben.2024.03.002
Reddy JV, Raudenbush K, Papoutsakis ET, Ierapetritou M. Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation. Biotechnol Adv. 2023 Oct;67:108179. doi: 10.1016/j.biotechadv.2023.108179. Epub 2023 May 29. PMID: 37257729.
Naik HM, Kumar S, Reddy JV, Gonzalez JE, McConnell BO, Dhara VG, Wang T, Yu M, Antoniewicz MR, Betenbaugh MJ. Chemical inhibitors of hexokinase-2 enzyme reduce lactate accumulation, alter glycosylation processing, and produce altered glycoforms in CHO cell cultures. Biotechnol Bioeng. 2023 Sep;120(9):2559-2577. doi: 10.1002/bit.28417. Epub 2023 May 6. PMID: 37148536.
Uprety BK, Reddy JV, Dalli SS, Rakshit SK. Utilisation of microbial oil obtained from crude glycerol for the production of polyol and its subsequent conversion to polyurethane foams. Bioresour Technol. 2017 Jul;235:309-315. doi: 10.1016/j.biortech.2017.03.126. Epub 2017 Mar 24. PMID: 28371769.